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PROJECT 6
WASTE MANAGEMENT RESEARCH CENTER (WMRC)
ORGANIC CHEMISTRY FIELD TRIP
(100 points)
PARKLAND | M
COLLEGE | A | F
| T | I
| T | R
| I | S
| S | T
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|______________________________| S
UNIVERSITY | T
| R
________________| E
FLORIDA AVENUE | E
___________________| T
|HAZELWOOOD DRIVE
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"LAB"
(PARK IN CIRCLE DRIVE)
PROCEED SOUTH ON MATTIS TO UNIVERSITY. TURN LEFT ON UNIVERSITY AND PROCEED
EAST ON UNIVERSITY TO FIRST. TURN RIGHT ON FIRST AND PROCEED SOUTH
ON FIRST PAST FLORIDA AVENUE UNTIL YOU GET TO HAZELWOOD DRIVE. TURN RIGHT
ON HAZELWOOD DRIVE FOR A SHORT DISTANCE UNTIL YOU SEE A CIRCLE DRIVE ON YOUR
LEFT! PARK IN THE CIRCLE DRIVE!! YOU HAVE 30 MINUTES TO GET TO THE LAB. YOU
SHOULD HAVE 30 MINUTES TO RETURN.
Organize a report on this organic chemistry field trip in the following way:
Give a brief explantion of what you learned at the three station presentations
you attended. Be concise and accurate. Be sure to cover the "SALIENT" features.
Be complete. Provide details using appropriate terminology.
STATIONS
1. ORGANIC PREPARATION (OP)
2. LIQUID CHROMATOGRAPHY (LC)
3. GAS CHROMATOGRAPHY (GC)
E-mail this assignment to me in two weeks!! The report should be a minimum of 500 words.
As a backup you must print out a hardcopy of the e-mail (complete with the exact
time and date that you e-mailed the project) and keep it in a folder for future use
in the event that the e-mail did not go through. This is required of every student!!
You may share notes to some degree. However, you may not do a joint assignment with any
other student.
Here is a list of terms that I hope you will discuss with your fellow students.
I would like to see you address these terms in your assignment.
Analyte - That component of sample you seek to isolate and measure the [ ] of.
Analytical spike - A quality assurance tool; an aliquot of the sample is amended with
a known amount of analyte(s) and the sample is processed to determine how much
of the amendment is recovered.
Linear Range - Quantifying the amount of analyte present by many analytical techniques
presumes that there is a linear relationship between analyte concentration and
analytical(instrument) response. The concentration range in which this occurs
is the linear range.
Beer's Law - A representation of linear performance in spectroscopy, Beer's Law states
that light Absorbance equals molar absorptivity xs path length times
concentration.
Nebulizer - Device on the atomic absorption spectrometer that delivers a fine mist of
sample to the flame.
Venturi Effect - The suction process that occurs when a gas flows across a small
orifice, this is used in atomic absorption spectroscopy to draw sample into
the nebulizer.
Digestion - Sample preparation with acid and heat that liberates metals and other
inorganic analytes from the sample matrix and puts them into the liquid phase.
Extraction - Sample preparation procedure in which an appropriate solvent is used to
move organic analytes from the sample matrix into the solvent phase.
Separatory Funnel - Tapered "flask" used to extract water samples, the funnel shape
allows one to effectively isolate the aqueous and organic phases.
Soxhlet Extractor - Extraction device that is used to repeatedly reflux an organic
solvent through a solid sample.
Supercritical fluid - A gas under some critical temperature and pressure that may be
used as an extraction solvent.
Chromatography - The science of using separation techniques to pull apart components
of a complex mixture, allowing further characterization of those components.
Injector - The "port" on a chromatograph that facilitates introduction of the sample
onto the column.
Detector - The device at the exit end of the chromatography column that responds to at
least some of the analytes being separated.
Retention Time - The characteristic time at which an analyte appears in the
chromatography process - assume instrument conditions are maintained.
Peak - The detector response to an analyte coming off the chromatographic column.
The peak occurs at a characteristic "retention time" for a given analyte.
Eluent - The "mobile phase" in liquid chromatography, this is the liquid or liquid
mixture that moves the analytes through the column to the detector.
Isocratic - A liquid chromatographic condition in which the same solvent (eluent)
composition is used throughout the duration of the analytical run.
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